Custom RNA Synthesis Core
Precision in vitro transcription (IVT) and high-throughput automated production.
Overview
The RNA Synthesis Core offers highly customizable RNA synthesis by in vitro transcription (IVT). We cater to a wide range of basic and translational research needs, from small-scale template pilots to massive production runs, ensuring high purity, stability, and customized modifications for in-cell and in vivo studies.
Capabilities & Scales
We synthesize RNA from 20 nt to 10 kb in length, with flexible product yields scaling from 1 µg up to 10 mg. We also offer comprehensive IVT template design and synthesis services.
Scale: 1 µg – 10 mgEngineered to increase translation efficiency and reduce immunogenicity. We offer a wide choice of 5' cap structures, custom poly(A) tail lengths, and internal base modifications optimized for therapeutic vectors.
Powered by the Telesis BioxP instrument, our core can perform simultaneous, automated synthesis of up to 96 standard RNAs or capped/polyadenylated mRNAs with robust yield guarantees.
Throughput: 96-well parallel synthesisQuality Control & Deliverables
Every custom synthesis project undergoes rigorous quality assurance to verify sequence integrity, exact concentration, and structural purity before handoff. We can accommodate specialized purification protocols upon request.
Upon project completion, researchers receive their purified RNA alongside a comprehensive QC specification report ensuring the product meets all downstream application requirements:
| Target Construct | Length (nt) | Concentration (ng/µL) | Total Yield (µg) | A260/280 | Purity Status |
|---|---|---|---|---|---|
| GFP_Control_mRNA_Cap1 | 950 | 1,205.4 | 60.2 | 2.08 | PASS |
| Therapeutic_Vector_ModA | 2,100 | 850.0 | 42.5 | 2.10 | PASS |
Project Workflow
From initial sequence design to final physical handoff, our process ensures precision at every step.
Consultation
Discuss sequence design, required modifications, and target yields.
Template Prep
Design and synthesis of custom IVT templates for your sequence.
Synthesis
Automated or manual in vitro transcription and capping.
Purification & QC
Rigorous purity checks via spec and fragment analysis.
Handoff
Delivery of stable, purified RNA and comprehensive QC reports.
- Consultation: Discuss design and modifications.
- Template Prep: Custom IVT template generation.
- Synthesis: Transcription and processing.
- Purification & QC: Integrity and yield verification.
- Handoff: Delivery of RNA and final reports.
Acknowledgment Policy
Standard Language: "We thank the UC San Diego Center for RNA Technologies and Therapeutics for custom RNA synthesis services."